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Λέκκα Μαρία – Ελένη

 

 

 

MARILENA E. LEKKA

BRIEF CURRICULUM VITAE

 

 

 

 

NAME:                                    Marilena E. Lekka

Adress:                                 Division of Organic Chemistry and Biochemistry,                                                           Chemistry Dept. University of Ioannina,                                                                         45110, Ioannina- Greece                                                                                              Tel. +30 651 98367 or 98373  FAX +30 651 47832

PLACE,-DATE OF BIRTH:    Kos, Dodecanese, Greece, 31-12-1954

 

EDUCATION, PROFESSIONAL STATUS

Undergraduate and Post-graduate Studies

  • Graduation from the Chemistry Dept. Of the Aristotelean University of Thessaloniki (1977)
  • PhD in Biochemistry from the Chemistry Dept. of the University of Ioannina- Greece (1987)
  • Short-term scientific mission at INSERM U 200, Paris-France, (1 Aug.-30 Sept. 1988). Director: Dr. J. Benveniste, Subject: «Biosynthetic enzymes of PAF»
  • Sabbatical leave: Dept. of Biochemistry, University of Texas at San Antonio, Health Science Center, Director: Professor D.J. Hanahan (March 1989- August 1990). Subject: «Molecular characterization of platelet-activating factor inhibitors».
  • Short-term scientific mission at the Physiology Dept. of Kuopio, Finland, (1 Aug.-15 Sept 1994). Director: Dr. O. Hanninen. Subject: «Biotransformation of xenobiotics».
  • Invited Professor at the Lab. of Membrane Biogenesis of the University Victor Segalen , Bordeaux-2 . Director: c. cassagne. Subject : “Detection of antilipid autoantibodies in bronchoalveolar lavage fluid” and “Affinity chromatography for the analysis of IgGs” (1997, 1998 and 2001, 2 months per year)

Professional status

  • Responsible for the quality control at the factory of raw materials "Koropoulis A.E." Attiki, Greece, (1979-1980).
  • Research associate in the Dept. of Chemistry, Lab. of Biochemistry of the University of Ioannina-Greece, (1980-1989).
  • Lecturer in the Division of Organic Chemistry and Biochemistry of the University of Ioannina, Greece, (1989 - 1993).
  • Assistant Professor in the Division of Organic Chemistry and Biochemistry of the University of Ioannina, Greece, (1993- today).

 

 

INSTRUCTION

 

  1. Undergraduate Programme of Chemistry Dept. Univ. Ioannina
  • “Laboratory Exercises in basic biochemistry”:     1980-today
  • “Basic concepts in Biochemistry”:                                   1997-today
  • “Biological Membranes and Cell Signalling”:       1993-2000
  1. Funded undergraduate programmes in the Univ. Ioannina

“Laboratory Exercises in basic biochemistry”:                    1980-2000

  1. Graduate Programme of Chemistry Dept. Univ. Ioannina
  • “Biological Membranes and Cell Signalling”:       1997-today
  • “Chromatographic Methods of Analysis”:                       1997-2000
  • “Radiometric methods” of analysis:                                             1997-today

 

 

Research Projects

 

ΔΙΑΚΡΙΣΕΙΣ ΕΡΓΑΣΙΩΝ

 

  1. AC Lachana, VE Mitsi-Malamou, L. Pappa, M. Machera, S. Liokatis, J. Pnevmatikos, ME Lekka, G. Nakos “Immunoparalysis in ventilator-associated pneumonia” 5th State of the Art Interdisciplinary Review Course. Athens, April 6-8, Athens (3rd Prize)
  2. G. Nakos, V. Mitsi, K. Karassavoglou, A. Lachana,and M. Lekka (2002) Immunoparalysis in patients with severe trauma and the effect of inhaled interferon Crit. Care Med. 30 (7): 1488-1494): Editorial των D.J. Dries and J.F. Perry, Jr.: Interferon-γ: titration of inflammation. Crit. Care Med. (2002), 30, 1663-1664
  3. Γ. Κωσταπαναγιώτου, Χ. Ρούτση, Β. Σμυρνιώτης, Ι. Χονδρουδάκη, Ει. Κητσιούλη, Μ.Ε. Λέκκα, Ν. Ερκαδόπουλος, Γ. Νάκος «Μεταβολές του βρογχοκυψελιδικού εκπλύματος κατά την κεραυνοβόλο ηπατική ανεπάρκεια». Poster 8ο Πανελλήνιο Συνέδριο Εντατικής θεραπείας, Αθήνα 19-21 Οκτ. 2001, (3o βραβείο)
  4. G. Nakos, Ei. Kitsiouli and M.E. Lekka. “Phospholipase A2 and PAF-acetylhydrolase levels and distribution in BAL fluid of patients with ARDS”.  6th State-of-the-Art Interdisciplinary Review Course, Αθήνα, 12-14 Απριλίου: (1st Prize)
  5. Phospholipases A2 levels and distribution in bronchoalveolar lavage fluid of patients with Acute respiratory Distress Syndrome (ARDS): Application of a fluorimetric method: Εθνικό βραβείο Roche 2002 / International Federation of Clinical Chemistry (IFCC)-
  6. Phospholipases A2 levels and distribution in bronchoalveolar lavage fluid of patients with Acute respiratory Distress Syndrome (ARDS): Application of a fluorimetric method. Bραβείο Roche 2002 / International Federation of Clinical Chemistry (IFCC)-: Επιλογή μεταξύ των 10 πρώτων από 30 χώρες

 

Recognition

1.Reviewer in

  • “Life Sciences”
  • “Biochim. Biophys. Acta”
  • “U.S. Department of Energy”
  1. Invited professor in Victor Segalen Bordeaux-2

 

PATENT

Lekka Maria-Eleni, Karkabounas Athanassios, University of Ioannina Research Committee, «Immobilization of lipid substrates in order to develop and evaluate a fluorimetric assay for the determination of phospholipase A2 activity in biological samples ». ΟΒΙ, GR1007801 (2011)

 

 

PUBLICATIONS

  1. Lekka, M., A.D. Tselepis, and D. Tsoukatos, (1986). 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (PAF) is a minor lipid component in Tetrahymena pyriformis cells: FEBS Letters, 208, 52-54.
  2. Lekka, M., D. Tsoukatos, A.D.Tselepis, and V.M. Kapoulas, (1988). Semisynthetic preparation of 1-O-hexadecyl-2-acetyl-sn-glyceryl-3-phosphocho-line (Platelet Activating Factor): Zeitchrift fur Naturforschung, 43C, 665-670.
  3. Lekka, M, D. Tsoukatos, and V.M. Kapoulas, (1989). Uptake of Platelet-Activating Factor (1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) by Tetrahymena pyriformis: Comp. J. Biochem. Physiol., 93B(1), 113-117.
  4. Kolios, M. Lekka, M.A. Typas, and C. Drainas, (1989). Bioconversion of fruit and sugar beet extracts by Zymomonas mobilis to ethanol and other fine chemicals». J. Ferm. Bioeng., 67(5), 363-365.
  5. Lekka, M., D. Tsoukatos, and V.M. Kapoulas, (1990). In vivo metabolism of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphocholine) by the protozoan Tetrahymena pyriformis: Biochim. Biophys. Acta, 1042(2), 217-220.
  6. Tselepis, M.E. Lekka, and D. Tsoukatos, (1991). A PAF-acetylhydrolase activity in Tetrahymena pyriformis cells. FEBS Letters, 288, 147-150
  7. Lekka, M., Tokumura, A., Tsuji, H. and Hanahan, D.J. (1993). "Isolation of a phospholipid inhibitor of platelet activating factor-induced activity from perfused rat liver: Identification as phosphatidylglycerol". Arch. Biochem. Biophys. 302, 380-384
  8. Tsoukatos, D., Tselepis, A.D. and Lekka, M.E. (1993) "Studies on the subcellular distribution of 1-O-alkyl-2-acetyl-sn-glycerophosphocholine (PAF) and on the enzymic activities involved in its biosynthesis within the ciliate Tetrahymena pyriformis". Biochim. Biophys. Acta, 1170, 258-264.
  9. Segerer, H., Scheid, A., Wagner, MH., Lekka, M. and Obladen, M. (1996) "Rapid tracheal infusion of surfactant versus bolus instillation in rabbits: effects on oxygenation, blood pressure, and surfactant distribution». Biol. Neonate, 69, 119-127.
  10. Tellis, K., Tsoukatos, D. and Lekka, ME (1996). "Isolation, chemical characterization and subcellular distribution of 1-O-alkyl-2-acetyl-sn-glycerol in Tetrahymena pyriformis  cells". J. Biochem. 119, 823-827.
  11. Nakos, G., Pneumatikos, J., Tsangaris, H, Tellis, K and Lekka, M.E. (1997) «Protein and phospholipids in BAL from patients with hydrostatic pulmonary edema» Am. J. Resp. Crit. Care Med. 155, 945-951
  12. Nakos, G., Kitsiouli, E.I., Tsangaris, I, and Lekka, M.E. (1998) «Bronchoalveolar lavage fluid characteristics of early, intermediate and late phases of ARDS» Intensive Care Med. 24, 296-303
  13. Nakos, G., Kitsiouli, E.I., and Lekka, M.E. (1998) “Bronchoalveolar lavage alterations in pulmonary embolism” Am. J. Resp. Crit. Care Med 158, 1504-1510.
  14. Kitsiouli E.I., Nakos G., and Lekka M.E. (1999) “Differential determination of phospholipase A2 and PAF-acetylhydrolase in biological fluids using fluorescent substrates”. J. Lipid Res. 40, 2346-2356.
  15. Tellis, C and M. E. Lekka (2000) 1–O–alkyl–2–acetyl–sn-glycerophosphate: phosphohydrolase activity in Tetrahymena pyriformis. J. Eukaryot. Microbiol. 47(2), 122-128.
  16. E. Przsybos and M. Lekka (2000) Studies on the Paramecium aurelia species complex in Lake Pamvotis (Ioannina, N.W. Greece) Folia Biologica (Krakow), 48, 61-63
  17. G. Nakos, E.I. Kitsiouli, L. Maneta-Peyret, C. Cassagne, E. Tsianos, and M. Lekka. (2001) The characteristics of bronchoalveolar lavage from patient with antiphospholipid syndrome who developed acute respiratory distress syndrome. J. Clin. Rheumatol. 20: 91-97.
  18. L. Maneta-Peyret, E.I. Kitsiouli, G. Nakos, M. Lekka and C. Cassagne (2001) “Auto-antibodies to lipids in bronchoalveolar lavage fluid of patients with adult respiratory distress syndrome” Crit. Care Med., 29, 1950-1954
  19. G. Nakos, V. Mitsi, K. Karassavoglou, A. Lachana,and M. Lekka (2002) Immunoparalysis in patients with severe trauma and the effect of inhaled interferon Crit. Care Med. 30 (7): 1488-1494)
  20. T.A. Albanis, D. Bochicchio, S.A. Bufo, I. Cospito, M. D’Auria, M. Lekka and L. Scrano (2002) «Surface adsorption and photo-reactivity of sulfonurea herbicides» Intern. J. Environ. Anal. Chem.
  21. Ei. Kitsiouli, M.E. Lekka, G. Nakos, C. Cassagne ans L. Maneta-Peyret (2002) “Lipids are co-eluted with immunoglobulins G during purification by recombinant streptococcal protein G-affinity chromatography” J. Immunological Methods (In press)

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  1. Nakos, G., Tsangaris, H. Liokatis, S., Kitsiouli, Ei., Lekka M.E. “Ventilator-Associated Pneumonia and atelectasis: Evaluation through Bronchoalveolar Lavage Fluid analysis”.
  2. G. Kostopanagiotou, C. Routsi, V. Smyrniotis M. E. Lekka, E. Kitsiouli, N. Arkadopoulos and G. Nakos, (2002) “Alterations in bronchoalveolar lavage fluid during fulminant hepatic failure” J. Hepatology (submitted)
  3. Phospholipases A2 levels and distribution in bronchoalveolar lavage fluid of patients with Acute respiratory Distress Syndrome (ARDS): Application of a fluorimetric method. (submitted)

 

Congresses

 

  1. Lekka, D. Tsoukatos, C.A. Demopoulos and V.M. Kapoulas. "Uptake of AGEPC by Tetrahymena pyriformis". New Horizons in Platelet Activating Factor Research. Hilton Head Island, S. Carolina, USA, Oct. 15-18, 1985, Abstract book p.53
  2. Lekka, D. Tsoukatos, C.A. Demopoulos and V.M. Kapoulas. "Effects of AGEPC on the fatty acid composition of the lipids of Tetrahymena pyriformis". New Horizons in Platelet Activating Factor Research. Hilton Head Island, S. Carolina, USA, Oct. 15-18, 1985, Abstract book, p.54
  3. Lekka, D. Tsoukatos, and A.D. Tselepis. "Semisynthetic preparation of  PAF from the protozoan  T. pyriformis".3Ô Panhellenic Symposium of Pharmacochemistry. Oct. 1986, pp. 25-30 Thessaloniki, Greece,
  4. Lekka, A.D. Tselepis and D. Tsoukatos,. "Biosynthesis of PAF within the protozoan T. pyriformis". «Chimica Chronica, New Series, pp. 358-361 (1986) 11Ô Panhellenic Congress of Chemistry, "Chemistry and Health" Greek Chemists Association, Athens, 2-5 Dec. 1986.
  5. Kolios, M. Lekka, C. Drainas and M.A. Typas. "Growth rate and ethanol production of Zymomonas mobilis on fruit and sugar beet extracts" Biotechnology Action Potential (BAP), "Culture Collections and genetic engineering of microorganisms". Ioannina 23-25 April 1987, pp.88-89.
  6. Lekka, D. Tsoukatos, A.D. Tselepis and V.M. Kapoulas "Biosynthesis and degradation of PAF within the protozoan T. pyriformis".1st Panhellenic Symposium on PAF. 28 Nov. 1987, Ioannina, Greece. Proceedins, pp. 67-71.
  7. Lekka, D. Tsoukatos, A.D. Tselepis, and V.M. Kapoulas. "Tetrahymena pyriformis : A useful model for studying the physiological production and role of Platelet-Activating Factor (PAF) in the eukariotic cell". "Biochemistry and Biophysics Newsletter, Dec 1989, pp. 62-63. 32th Scientific Conference of the Hellenic Biochemical and Biophysical Society. Ioannina, June 2-3, 1989.
  8. Tsoukatos, A.D. Tselepis, and M. Lekka,. "Biosynthesis and degradation of Platelet-Activating factor (PAF) by subcellular fractions of the protozoanTetrahymena pyriformis "  "Biochemistry and Biophysics Newsletter, vol. 33, Oct. 1991, pp. 38-41. 36th Scientific Conference of the Hellenic Biochemical and Biophysical Society. Crete, June 7-8, 1991.
  9. Tsoukatos, A.D. Tselepis and M.E. Lekka. "Localization and biosynthesis of PAF in subcellular fractions of Tetrahymena pyriformis". Fourth International Congress on PAF and Related Lipid Mediators. Salt Lake City, Utah, USA, Sept. 22-25, 1992, Abstract book p.29, No PO1.35
  10. Lekka, K. Tellis, J. Pnevmatikos, M. Guillaumme, and G. Nakos. "Biochemical parameters in adult respiratory distress syndrome". Annual Congress of the "European Respiratory Society" Barcelona, Spain, Sept. 1995. Eur. Res. Abstract book J 8 (S19), p.86 (1995)
  11. Tellis, D.C. Tsoukatos and M.E. Lekka "PAF biosynthesis in T. pyriformis: The last step of the de novo pathway" 5th Int. Congress on Platelet-activating factor and related lipid mediators. Berlin, 12-16 Sept 1995. Αbstract book P.136
  12. Lekka ME., Tellis K., Pneumaticos J.,  Dalavaga Y., G. Nakos. "Biochemical Parameters in BAL from patients with Hydrostatic  Pulmonary Edema".
  13. 5th Int. Congress on Platelet-activating factor and related lipid mediators. Berlin, 12-16 Sept 1995. Αbstract book P.130
  14. Lekka and D. Tsoukatos. "Occurence of 1-O-alkyl-2-acetyl-sn-glycerol in Tetrahymena pyriformis». "Biochemistry and Biophysics Newsletter", vol. 37, Jan. 1995, pp. 58-60. 41st Scientific Conference of the Hellenic Biochemical and Biophysical Society. Athens, January 21-22, 1994.
  15. Lekka, J. Pnevmatikos and G. Nakos. "Acetylhydrolase activity in bronchoalveolar lavage of patients with adult respiratory destress syndrome" "Biochemistry and Biophysics Newsletter", vol. 38, March 1995, p. 96. 42nd Scientific Conference of the Hellenic Biochemical and Biophysical Society. Athens, January 13-14, 1995.
  16. Tsangaris, H., Lekka ME, and G. Nakos. «Effect of mechanical ventillation on lung surfactant»
  17. 8th PanhellenicMedical Congress of Chest Disease.  Athens,, 14-17 March, 1996, Abstract book,  P.10
  18. 8. Tsangaris H., Kitsiouli E. I., Nathanail C., Lekka M.E., Nakos G. “The effect of ventilator-associated pneumonia on protein and phospholipids from BAL” Oral presentation in the Annual Congress of the American Society of Chest Disease, San Francisco, 27-31 Oct. 1996
  19. 9. Tsangaris H., Kitsiouli E. I., Koulouras V., Nakos G., Lekka M.E. “BAL alterations in pulmonary embolism” Oral presentation in the 10th annual congress of the European Society of Intensive Care Medicine, Paris, France 7-10 Sept 1997.
  20. 10. Kitsiouli E. I., Nakos G., Lekka M. E. “Fluorimetric detection of phospholipase A2 (Plase A2) in human bronchoalveolar lavage (BAL)” Poster presentation in the 38th International Conference on the Biochemistry of Lipids (ICBL), September 16-19, 1997, Assisi, Italy.
  21. Kitsiouli E. I., Nakos G., Lekka M. E. “Application of a fluorimetric assay for phospholipase A2 bronchoalveolar lavage (BAL) fluid” Poster presentation in the 5th Meeting of the Balkan Clinical Laboratory Federation (BJCL), 9-12 October, 1997, Ioannina, Greece.
  22. Kitsiouli E. I., Nakos G., Lekka M. E. “Fluorimetric Determination of phospholipase A2 and PAF-acetylhydrolase: effect of albumin” Oral presentation. Int. Conf. On “Lipases and Lipids: Structure, Function and Biotechnological Applications, May 6-8 1999, Santorini, Hellas
  23. Kitsiouli E. I., Nakos G., Lekka M. E. “PAF-acetylhydrolase activity in Bronchoalveolar lavage fluid: fluorimetric detection and specific inhibitors” Poster Session. Int. Conf. On Phospholipases A2. May 26-29 1999, Berlin Germany
  24. Kitsiouli E. I., Karasavoglou A., Lachana C. A., Malamou-Mitsi V. D., Nakos G., M.E. Lekka. Local increase of PAF and phospholipases in bronchoalveolar lavage fluid after inhalation of interferon. Poster presentation in the 40th International Conference on the Biochemistry of Lipids (ICBL), 14-17 September 1999, Dijon, France.
  25. 11. Kitsiouli E. I., Nakos G., Lekka M. E. “Application of a fluorimetric assay for phospholipase A2 bronchoalveolar lavage (BAL) fluid” Poster presentation in the 5th Meeting of the Balkan Clinical Laboratory Federation (BJCL), 9-12 October, 1997, Ioannina, Greece.
  26. Kitsiouli, E. I., Karasavoglou A., Lachana C. A., Malamou-Mitsi V. D., Nakos G., M.E. Lekka. Local increase of PAF and phospholipases in bronchoalveolar lavage fluid after inhalation of interferon. Newsletter p. 43-47, Proceedings of the 51st meeting of the Hellenic Society of Biochemistry & Molecular Biology, Oral presentation, 9-11 December 1999, Athens.
  27. Kitsiouli, E. I., L. Maneta-Peyret, C. Cassagne, G. Nakos, M. E. Lekka. Protein G-sepharose affinity column retains lipids. Proceedings of the 51st meeting of the Hellenic Society of Biochemistry & Molecular Biology, Poster presentation, 9-11 December 1999, Athens

 

 

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